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SibEnzyme Ltd. :: Display product information: AspS9 I
Name
AspS9 I
Catalog numberE117E118
Package, u.a.10005000
Concentration, u.a./ml10000-3000010000-30000

Recognition siteG^GNCC
CCNG^G
Methylation sensitivityBloked by overlapping Dcm methylation(CmCWGG): GGNCCWGG
OriginArthrobacter species S9
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 30 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
50 - 7550 - 7575 - 10010050 - 75

References: Dedkov, V.S., Kileva, E.V., Shevchenko, A.V., Degtyarev, S.Kh. Unpublished observations (1995).