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Name AspS9 I Catalog number E117 E118 Package, u.a. 1000 5000 Concentration, u.a./ml 10000-30000 10000-30000 Recognition site G^GNCC CCNG^G Methylation sensitivity Bloked by overlapping Dcm methylation(CmCWGG): GGNCCWGG Origin Arthrobacter species S9 Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20°C. Assayed on Lambda DNA Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. Non-specific activity No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 30 u.a. of enzyme for 16 hours at 37°C. Optimum temperature 37 oC Inactivation 20 minutes 65oC Optimum SE-buffer W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Enzyme % activity: B G O W Y 50 - 75 50 - 75 75 - 100 100 50 - 75 References: Dedkov, V.S., Kileva, E.V., Shevchenko, A.V., Degtyarev, S.Kh. Unpublished observations (1995).