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SibEnzyme Ltd. :: Display product information: AspLE I
Catalog numberE221E222
Package, u.a.5002500
Concentration, u.a./ml10000-3000010000-30000

Recognition siteGCG^C
Methylation sensitivityBlocked by
5'-G(5mC)GC-3'/3-CG(5mC)G-5' or
5'-G(5mC)GC-3'/3'-CGCG-5' methylation.
Not blocked by
5'-GCG(5mC)-3'/3-(5mC)GCG-5 or
5'-GCG(5mC)-3'/3'-CGCG-5' methylation.
Cut hemimethylated site: 5'-G(5mC)GC-3'/3'-CGCG-5'
OriginArthrobacter species LE3860
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
10 - 2575 - 10010050 - 7525 - 50

References: Vysotskaya, E.M., Gonchar, D.A., Shevchenko, A.V., Abdurashitov, M.A., Degtyarev, S.Kh. Unpublished observations (1998).