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SibEnzyme Ltd. :: Display product information: EcoICR I
Name
EcoICR I
Catalog numberE469E470
Package, u.a.2001000
Concentration, u.a./ml2000-100002000-10000

Recognition siteGAG^CTC
CTC^GAG
Methylation sensitivitynot tested
OriginEscherichia coli ICR
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20C.
Assayed onLambda DNA (HindIII-digest)
LigationAfter 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
75 - 1001000 - 100 - 1075 - 100

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml
Do not use BSA for long incubation.
References: Sugisaki H., Maekawa Y., Kanazawa S., Takanami M. Bull. Inst. Chem. Res. Kyoto Univ. 60: 328-335 (1982). Yankovsky N.K., Fonstein M.Yu., Lashina S.Yu., Bukanov N.O., Yakubovich N.V., Ermakova L.M., Rebentish B.A., Janulaitis A., Debabov V.G. Gene 81: 203-210 (1989).