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Name BstX I Catalog number E465 E466 Package, u.a. 200 1000 Concentration, u.a./ml 5000-15000 5000-15000 Recognition site CCANNNNN^NTGG GGTN^NNNNNACC Methylation sensitivity not tested Origin Bacillus stearothermophilus X Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20°C. Assayed on Lambda DNA Ligation After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut. Non-specific activity No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. Optimum temperature 37 oC Inactivation 20 minutes 65oC Optimum SE-buffer O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Enzyme % activity: B G O W Y 10 - 25 10 - 25 100 75 - 100 25 - 50 References: Wise, R., Schildkraut, I. Unpublished observations (1992).