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SibEnzyme Ltd. :: Display product information: BstX I
BstX I
Catalog numberE465E466
Package, u.a.2001000
Concentration, u.a./ml5000-150005000-15000

Recognition siteCCANNNNN^NTGG
Methylation sensitivitynot tested
OriginBacillus stearothermophilus X
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
10 - 2510 - 2510075 - 10025 - 50

References: Wise, R., Schildkraut, I. Unpublished observations (1992).