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SibEnzyme Ltd. :: Display product information: Hpa I
Name
Hpa I
Catalog numberE077E078
Package, u.a.5002500
Concentration, u.a./ml50005000

Recognition siteGTT^AAC
CAA^TTG
Methylation sensitivitynot tested
OriginE.coli strain, that carries the cloned gene Hpa I from Haemophilus parainfluenzae
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme about 60% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
BGOWY
0 - 1050 - 7510 - 2525 - 50100

Note: High enzyme concentration results in star activity.
References: Sharp, P.A., Sugden, B., Sambrook, J. Biochemistry 12: 3055-3063 (1973). Garfin, D.E., Goodman, H.M. Biochem. Biophys. Res. Commun. 59: 108-116 (1974).