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SibEnzyme Ltd. :: Display product information: AspA2 I
Name
AspA2 I
Catalog numberE245E246
Package, u.a.5002500
Concentration, u.a./ml10000-2000010000-20000

Recognition siteC^CTAGG
GGATC^C
Methylation sensitivitynot tested
OriginArthrobacter species A2
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA (HindIII-digest)
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
10 - 2550 - 7575 - 10010075 - 100

Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References: Abdurashitov, M.A., Dedkov, V.S., Shinkarenko, N.M., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2003).