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SibEnzyme Ltd. :: Display product information: BpmI
Name
BpmI
Catalog numberE467E468
Package, u.a.50250
Concentration, u.a./ml200200

Recognition siteCTGGAG(N)16^
GACCTC(N)14^
Methylation sensitivitynot tested
OriginBacillus pumilus
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20C
Assayed onLambda DNA
LigationAfter 2-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and 95% may be recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 0.4 u.a. of enzyme for 1 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
25 - 5050 - 7575 - 10010050 - 75

Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Degtyarev, S.Kh., Morgan P. Unpublished observation (1992).