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SibEnzyme Ltd. :: Display product information: BstAU I
Catalog numberE267E268
Package, u.a.5002500
Concentration, u.a./ml1000010000

Recognition siteT^GTACA
Methylation sensitivitynot tested
OriginBacillus stearothermophillus AU
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 ug/ml BSA; 50% glycerol. Store at -20°C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
10 - 2550 - 7525 - 5010025 - 50

References: Abdurashitov, M.A., Dedkov, V.S., Shinkarenko, N.M., Tomilova, J.E., Degtyarev, S.K. Unpublished observations (2003)