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SibEnzyme Ltd. :: Display product information: Hga I
Name
Hga I
Catalog numberE461E462
Package, u.a.50250
Concentration, u.a./ml10001000

Recognition siteGACGC(N)5^
CTGCG(N)10^
Methylation sensitivityBlocked by CG methylation.
OriginE.coli strain that carries the cloned HgaI gene from Haemophilus gallinarum
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -200C.
Assayed onDNA pBR322
LigationAfter 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityIncubation with > 2 units of HgaI per 1 ug of DNA and digestion > 1 hour are not recommended.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.)

Enzyme % activity:
BGOWY
10075 - 10010 - 2525 - 5050 - 75

References: Brown N.L., Smith M. Proc. Natl. Acad. Sci. U. S. A. 74: 3213-3216 (1977). Sugisaki H. Gene 3: 17-28 (1978).