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SibEnzyme Ltd. :: Display product information: BstMW I
Name
BstMW I
Catalog numberE459E460
Package, u.a.5002500
Concentration, u.a./ml2000-100002000-10000

Recognition siteGCNNNNN^NNGC
CGNN^NNNNNCG
Methylation sensitivitynot tested
OriginBacillus stearothermophilus MW
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 ug/ml BSA, 7 mM 2-mercaptoethanol; and 50% glycerol. Store at -20C. Store at -70ºC is recommended for period longer than 7 days.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 55C.
Optimum temperature55 oC
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
BGOWY
10 - 2525 - 5025 - 5050 - 75100

Note: At 37C activity is 20% from maximum
References: Nadeev A.N., Dedkov, V.S., Tomilova J.E., Degtyarev, S.K. Unpublished observations (2002).