Go to sibenzyme.com
SibEnzyme Ltd. :: Display product information: Ama87 I
Name
Ama87 I
Catalog numberE017E018
Package, u.a.10005000
Concentration, u.a./ml10000-3000010000-30000

Recognition siteC^YCGRG
GRGCY^C
Methylation sensitivitynot tested
OriginAlteromonas macleodii 87
Storage conditions10 mM KPO4 (pH 7.2); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
10 - 2550 - 7575 - 1001000 - 10

Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References: Degtyarev, S.Kh., Repin, V.E., Unpublished observations.