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Name Ama87 I Catalog number E017 E018 Package, u.a. 1000 5000 Concentration, u.a./ml 10000-30000 10000-30000 Recognition site C^YCGRG GRGCY^C Methylation sensitivity not tested Origin Alteromonas macleodii 87 Storage conditions 10 mM KPO4 (pH 7.2); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C. Assayed on Lambda DNA Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. Non-specific activity No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Optimum temperature 37 oC Inactivation 20 minutes 65oC Optimum SE-buffer W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA Enzyme % activity: B G O W Y 10 - 25 50 - 75 75 - 100 100 0 - 10 Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation. References: Degtyarev, S.Kh., Repin, V.E., Unpublished observations.