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SibEnzyme Ltd. :: Display product information: Psp6 I
Psp6 I
Catalog numberE453E454
Package, u.a.100500
Concentration, u.a./ml1000-30001000-3000

Recognition site^CCWGG
Methylation sensitivityBlocked by overlapping Dcm methylation(CmCWGG): CCWGG.
OriginPseudomonas species 6
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; and 50% glycerol.. Store at -20C.
Assayed onLambda DNA (dcm-)
LigationAfter 3-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 6 u.a. of enzyme for 16 hours at 37C.
Optimum temperature55 oC
Inactivation 20 minutes80oC
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.)

Enzyme % activity:
10050 - 7510 - 2525 - 5075 - 100

References: Dedkov, V.S., Tomilova, J.E., Kileva, E.V., Degtyarev, S.K. Unpublished observations. (2002)