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SibEnzyme Ltd. :: Display product information: Bmt I
Name
Bmt I
Catalog numberE457E458
Package, u.a.10005000
Concentration, u.a./ml2000020000

Recognition siteGCTAG^C
C^GATCG
Methylation sensitivitynot tested
OriginE.coli strain that carries the cloned Bmt I gene from Bacillus megaterium S2
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA (Hind III-digest)
LigationAfter 20-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
10 - 2550 - 7550 - 7510075 - 100

Note: The minimum number of units that resulted in complete digestion of 1 ug of substrate DNA in 16 hours is 0,13. BmtI cleaves linear plasmid DNA at a rate 5 times higher than supercoiled plasmid DNA.
References: Dedkov, V.S., Nayakshina, T.N., Popichenko, D.V., Degtyarev, S.K. Biotekhnologiya 1: 11-15 (2003).