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Name Bmt I Catalog number E457 E458 Package, u.a. 1000 5000 Concentration, u.a./ml 20000 20000 Recognition site GCTAG^C C^GATCG Methylation sensitivity not tested Origin E.coli strain that carries the cloned Bmt I gene from Bacillus megaterium S2 Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20°C. Assayed on Lambda DNA (Hind III-digest) Ligation After 20-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut. Non-specific activity No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Optimum temperature 37 oC Inactivation 20 minutes 65oC Optimum SE-buffer W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Enzyme % activity: B G O W Y 10 - 25 50 - 75 50 - 75 100 75 - 100 Note: The minimum number of units that resulted in complete digestion of 1 ug of substrate DNA in 16 hours is 0,13. BmtI cleaves linear plasmid DNA at a rate 5 times higher than supercoiled plasmid DNA. References: Dedkov, V.S., Nayakshina, T.N., Popichenko, D.V., Degtyarev, S.K. Biotekhnologiya 1: 11-15 (2003).