|Catalog number||E457||E458 |
|Package, u.a.||1000||5000 |
|Concentration, u.a./ml||20000||20000 |
|Methylation sensitivity||not tested|
|Origin||E.coli strain that carries the cloned Bmt I gene from Bacillus megaterium S2|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20°C.|
|Assayed on||Lambda DNA (Hind III-digest)|
|Ligation||After 20-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut.|
|Non-specific activity||No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.|
|Optimum temperature||37 oC|
|Inactivation 20 minutes||65oC|
|Optimum SE-buffer||W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)|
Enzyme % activity:
|10 - 25||50 - 75||50 - 75||100||75 - 100|
Note: The minimum number of units that resulted in complete digestion of 1 ug of substrate DNA in 16 hours is 0,13.
BmtI cleaves linear plasmid DNA at a rate 5 times higher than supercoiled plasmid DNA.
References: Dedkov, V.S., Nayakshina, T.N., Popichenko, D.V., Degtyarev, S.K.
Biotekhnologiya 1: 11-15 (2003).