Go to sibenzyme.com
SibEnzyme Ltd. :: Display product information: Hind II
Name
Hind II
Catalog numberE201E202
Package, u.a.10005000
Concentration, u.a./ml1000010000

Recognition siteGTY^RAC
CAR^YTG
Methylation sensitivitynot tested
OriginE.coli strain, that carries the cloned gene HindII from Haemophilus influenzae
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme 60% of the DNA fragments can be ligated and recut. In the presence of 10%PEG ligation is better.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
75 - 10010025 - 5025 - 5075 - 100

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Kelly, T.J. Jr., Smith, H.O. J. Mol. Biol. 51: 393-409 (1970).