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SibEnzyme Ltd. :: Display product information: FatI
Catalog numberE155E156
Package, u.a.100500
Concentration, u.a./ml30003000

Recognition site^CATG
Methylation sensitivityBlocked by mCATG methylation
OriginE.coli strain that carries the cloned Fat I gene from Flavobacterium aquatile NL3
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; and 50% glycerol. Store at -20C.
Assayed onpUC19 DNA
LigationAfter 2-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of DNA with 3 u.a. of enzyme for 16 hours at 55C.
Optimum temperature55 oC
Inactivation 20 minutes65oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)

Enzyme % activity:
10 - 2510025 - 5010 - 2550 - 75

Note: The minimum number of units that resulted in complete digestion of 1 ug of substrate DNA in 16 hours is 0,25.
FatI cleaves linear plasmid DNA at a rate 1.5-2 times higher than supercoiled plasmid DNA.
References: Dedkov, V.S., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K. Biotekhnologia #5, 3-7 (2002)