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SibEnzyme Ltd. :: Display product information: FalI
Catalog numberE153E154
Package, u.a.100500
Concentration, u.a./ml1000-30001000-3000

Recognition site^(N)8AAGNNNNNCTT(N)13^
Methylation sensitivitynot tested
OriginFlavobacterium aquatile Ob10
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 3-fold overdigestion with enzyme 20% of the DNA fragments can be ligated and of these 80% can be recut. In the presence of 10%PEG ligation is better.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + SAM

Enzyme % activity:
0 - 1025 - 5075 - 10010050 - 75

Note: High enzyme concentration results in star activity
To obtain 100% activity, SAM should be added to a final concentration 0.01 mM
References: Dedkov, V.S., Sinichkina, S.A., Abdurashitov, M.A., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).