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Name FalI Catalog number E153 E154 Package, u.a. 100 500 Concentration, u.a./ml 1000-3000 1000-3000 Recognition site ^(N)8AAGNNNNNCTT(N)13^ ^(N)13TTCNNNNNGAA(N)8^ Methylation sensitivity not tested Origin Flavobacterium aquatile Ob10 Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20°C. Assayed on Lambda DNA Ligation After 3-fold overdigestion with enzyme 20% of the DNA fragments can be ligated and of these 80% can be recut. In the presence of 10%PEG ligation is better. Non-specific activity No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C. Optimum temperature 37 oC Inactivation 20 minutes 65oC Optimum SE-buffer W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + SAM Enzyme % activity: B G O W Y 0 - 10 25 - 50 75 - 100 100 50 - 75 Note: High enzyme concentration results in star activity To obtain 100% activity, SAM should be added to a final concentration 0.01 mM References: Dedkov, V.S., Sinichkina, S.A., Abdurashitov, M.A., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).