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SibEnzyme Ltd. :: Display product information: BstKTI
Name
BstKTI
Catalog numberE151E152
Package, u.a.2001000
Concentration, u.a./ml2000-50002000-5000

Recognition siteGAT^C
C^TAG
Methylation sensitivityBlocked by overlapping Dam methylation (GmATC) GATC .
Not blocked by CG methylation.
Cut hemimethylated site: 5- GmATC-3 / 5-GATC-3
OriginBacillus stearothermophilus KT
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA (dam-)
LigationAfter 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
25 - 5050 - 7575 - 10010050 - 75

References: Nadeev A.N., Chernukhin V. A., Sevastyanova O.O., Tomilova J.E., Shinkarenko N.M., Evdokimov A.A., Degtyarev S. Kh. BstKTI, a New dam-Sensitive Neoschizomer of Restriction Endonuclease MboI, which is Able to Cleave Hemimethylated Substrate.// Biotekhnologia (Moscow), No.2, 5-10 (2006). (In Russian)