|Catalog number||E451||E452 |
|Package, u.a.||50||250 |
|Concentration, u.a./ml||1000||1000 |
|Methylation sensitivity||not tested|
|Origin||E.coli strain that carries the cloned Acu I gene from Acinetobacter calcoaceticus SRW4|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.|
|Assayed on||Lambda DNA|
|Ligation||After 2-fold overdigestion with enzyme about 80% of the DNA fragments can be ligated. Of these, 80% can be recut.|
|Non-specific activity||No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C.|
|Optimum temperature||37 oC|
|Inactivation 20 minutes||65oC|
|Optimum SE-buffer||Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA + SAM|
Enzyme % activity:
|25 - 50||50 - 75||50 - 75||75 - 100||100|
Note: High enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml and SAM should be added to a final concentration 0.01 mM
Do not use BSA for long incubation.
References: Degtyarev, S.Kh., Kileva, E.V., Dedkov, V.S. Unpublished observations (2001).