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SibEnzyme Ltd. :: Display product information: Acu I
Acu I
Catalog numberE451E452
Package, u.a.50250
Concentration, u.a./ml10001000

Recognition siteCTGAAG(N)16^
Methylation sensitivitynot tested
OriginE.coli strain that carries the cloned Acu I gene from Acinetobacter calcoaceticus SRW4
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 2-fold overdigestion with enzyme about 80% of the DNA fragments can be ligated. Of these, 80% can be recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA + SAM

Enzyme % activity:
25 - 5050 - 7550 - 7575 - 100100

Note: High enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml and SAM should be added to a final concentration 0.01 mM
Do not use BSA for long incubation.
References: Degtyarev, S.Kh., Kileva, E.V., Dedkov, V.S. Unpublished observations (2001).