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Name BstV1I Catalog number E303 E304 Package, u.a. 100 500 Concentration, u.a./ml 1000-2000 1000-2000 Recognition site GCAGC(N)8^ CGTCG(N)12^ Methylation sensitivity not tested Origin Bacillus stearothermophilus V1 Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C. Assayed on pBR322 DNA Ligation After 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. Non-specific activity No nonspecific activity was detected after incubation of 1 ug of DNA with 2 u.a. of enzyme for 16 hours at 55°C. Optimum temperature 55 oC Inactivation 20 minutes 80oC Optimum SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) Enzyme % activity: B G O W Y 75 - 100 100 75 - 100 75 - 100 75 - 100 Note: At 37°C activity is 10% from maximum. References: Dedkov, V.S., Muradov, S.V., Shinkarenko, N.M., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).