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SibEnzyme Ltd. :: Display product information: BstV1I
Name
BstV1I
Catalog numberE303E304
Package, u.a.100500
Concentration, u.a./ml1000-20001000-2000

Recognition siteGCAGC(N)8^
CGTCG(N)12^
Methylation sensitivitynot tested
OriginBacillus stearothermophilus V1
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20C.
Assayed onpBR322 DNA
LigationAfter 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of DNA with 2 u.a. of enzyme for 16 hours at 55C.
Optimum temperature55 oC
Inactivation 20 minutes80oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
75 - 10010075 - 10075 - 10075 - 100

Note: At 37C activity is 10% from maximum.
References: Dedkov, V.S., Muradov, S.V., Shinkarenko, N.M., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).