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SibEnzyme Ltd. :: Display product information: BstSCI
Catalog numberE307E308
Package, u.a.100500
Concentration, u.a./ml2000-50002000-5000

Recognition site^CCNGG
Methylation sensitivityBlocked by Dcm-methylation (CmCWGG) CCWGG.
OriginBacillus stearothermophilus SC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; and 50% glycerol. Store at -20C.
Assayed onLambda DNA (dcm-)
LigationAfter 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 3 u.a. of enzyme for 16 hours at 55C.
Optimum temperature55 oC
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
50 - 7550 - 7550 - 7550 - 75100

Note: At 37C activity is 10% from maximum.
References: Dedkov, V.S., Muradov, S.V., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).