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SibEnzyme Ltd. :: Display product information: BstC8I
Catalog numberE305E306
Package, u.a.2001000
Concentration, u.a./ml10000-2000010000-20000

Recognition siteGCN^NGC
Methylation sensitivitynot tested
OriginBacillus stearothermophilus C8
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 55C.
Optimum temperature55 oC
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
10 - 2525 - 5050 - 7575 - 100100

Note: At 37C activity is 50% from maximum.
References: Abdurashitov, M.A., Dedkov, V.S., Mikhnenkova, N.A., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).