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SibEnzyme Ltd. :: Display product information: Bpu10I
Name
Bpu10I
Catalog numberE149E150
Package, u.a.2001000
Concentration, u.a./ml50005000

Recognition siteCC^TNAGC
GGANT^CG
Methylation sensitivitynot tested
OriginE.coli strain, that carries recombinant plasmids
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20C
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme 80% of the DNA fragments can be ligated. Of these 90% can be recut. In the presence of 10% PEG ligation is better.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 5 u.a. of enzyme for 1 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferK (10 mM Tris-HCl (pH 7.6 at 250C); 10 mM MgCl2; 100 mM KCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
10 - 2525 - 5050 - 7550 - 7525 - 50

Note: High enzyme concentration may result in star activity or incomplete DNA cleavage. We recommend increasing incubation time instead of using an excess of Bpu10I.
The minimum number of units that resulted in complete digestion of 1 ug of substrate DNA in 16 hours is 0,5.
References: Degtyarev, S.Kh., Zilkin, P.A., Prihodko, G.G., Repin, V.E., Rechkunova, N.I. Mol. Biol. (Mosk) 23: 1051-1056 (1989).