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SibEnzyme Ltd. :: Display product information: Psr I
Psr I
Catalog numberE131E132
Package, u.a.100500
Concentration, u.a./ml1000-30001000-3000

Recognition site^(N)7GAACNNNNNNTAC(N)12^
Methylation sensitivitynot tested
OriginPseudomonas stutzeri N2
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA, 50% glycerol. Store at -20C.
Assayed onT7 DNA
LigationAfter 2-fold overdigestion with enzyme more than 70% of DNA fragments can be ligated. Of these 80% can be recut. In the presence of 10% PEG ligation is better.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of T7 DNA with 2 u.a. of enzyme for 16 hours at 30C.
Optimum temperature30 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA

Enzyme % activity:
10 - 2510 - 250 - 100 - 10100

Note: High enzyme concentration may result in star activity.
Incubation at 37C results in 20% activity.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Dedkov, V.S., Kileva, E.V., Abdurashitov, M.A., Gonchar, D.A., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).