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SibEnzyme Ltd. :: Display product information: Mab I
Name
Mab I
Catalog numberE121E122
Package, u.a.2001000
Concentration, u.a./ml1000-50001000-5000

Recognition siteA^CCWGGT
TGGWCC^A
Methylation sensitivityNot blocked by overlapping Dcm methylation (CmCWGG) ACCWGGT.
OriginMicrobacterium arborescens SE
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA, 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
25 - 5050 - 7575 - 10010050 - 75

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Nadeev, A.N., Tsaplin, M.M., Abdurashitov, M.A., Dedkov, V.S., Kashirina, J.G., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).