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SibEnzyme Ltd. :: Display product information: BstHH I
Catalog numberE143E144
Package, u.a.200010000
Concentration, u.a./ml5000050000

Recognition siteGCG^C
Methylation sensitivityBlocked by
5`-G(5mC)GC-3`/3-CG(5mC)G-5` or
5`-G(5mC)GC-3`/3`-CGCG-5` methylation.
Not blocked by
5`-GCG(5mC)-3`/3`-(5mC)GCG-5` or
5`-GCG(5mC)-3`/3`-CGCG-5` methylation.
OriginBacillus stearothermophilus HH
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA, 50% glycerol; Store at 20C
Assayed onLambda DNA
LigationAfter 40-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of DNA with 100 u.a. of enzyme for 16 hours at 65C.
Optimum temperature50 oC
Inactivation 20 minutesNo
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA

Enzyme % activity:
75 - 10050 - 7525 - 5050 - 75100

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml
Do not use BSA for long incubation.
References: Shapovalova, M.A., Abdurashitov, M.A., Tumanova, I.Y., Dedkov, V.S., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).