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SibEnzyme Ltd. :: Display product information: Ahl I
Ahl I
Catalog numberE173E174
Package, u.a.10005000
Concentration, u.a./ml10000-3000010000-30000

Recognition siteA^CTAGT
Methylation sensitivitynot tested
OriginAlteromonas haloplanktis SP
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onT7 DNA
LigationAfter 20-fold overdigestion with enzyme more than 90% of T7 DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of T7 DNA with 40 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutesNo
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.) + BSA

Enzyme % activity:
10075 - 10025 - 5025 - 5075 - 100

Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Myakisheva, T.V., Belichenko, O.A., Popichenko, D.V., Dedkov, V.S., Degtyarev, S.Kh. Unpublished observations (2000).