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SibEnzyme Ltd. :: Display product information: Pct I
Pct I
Catalog numberE045E046
Package, u.a.10005000
Concentration, u.a./ml10000-4000010000-40000

Recognition siteGAATGCN^
Methylation sensitivitynot tested
OriginPlanococcus citreus SM
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 ug/ml BSA; and 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
25 - 5050 - 7510075 - 10010 - 25

References: Dedkov, V.S., Nayakshina, T.N., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2000).