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SibEnzyme Ltd. :: Display product information: Mhl I
Name
Mhl I
Catalog numberE049E050
Package, u.a.5002500
Concentration, u.a./ml5000-100005000-10000

Recognition siteGDGCH^C
C^HCGDG
Methylation sensitivitynot tested
OriginMicrococcus halobius SD
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
10 - 2525 - 5075 - 10010010 - 25

References: Shapovalova, M.A., Abdurashitov, M.A., Dedkov, V.S., Kashirina, J.G., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2000).