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SibEnzyme Ltd. :: Display product information: BstPA I
Name
BstPA I
Catalog numberE299E300
Package, u.a.10005000
Concentration, u.a./ml20000-5000020000-50000

Recognition siteGACNN^NNGTC
CTGNN^NNCAG
Methylation sensitivitynot tested
OriginBacillus stearothermophillus PA
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 ug/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme less than 5% of the DNA fragments can be ligated
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 25C
Optimum temperature65 oC
Inactivation 20 minutesNo
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
BGOWY
50 - 7525 - 5050 - 7550 - 75100

Note: High enzyme concentration and incubation at 65C for 16 hours results in star activity.
References: Tomilova, J.E., Dedkov, V.S., Kileva, E.V., Popichenko, D.V., Degtyarev, S.Kh. Unpublished observations (2000).