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SibEnzyme Ltd. :: Display product information: BstEN I
Name
BstEN I
Catalog numberE103E104
Package, u.a.2001000
Concentration, u.a./ml2000-50002000-5000

Recognition siteCCTNN^NNNAGG
GGANNN^NNTCC
Methylation sensitivityNot blocked by overlapping Dcm methylation(CmCWGG): CCTGGNNNAGG,
CCTNNNCCAGG.
OriginBacillus stearothermophillus EM
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 ug/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 3-fold overdigestion with enzyme about 60% of the DNA fragments can be ligated. Of these 90% can be recut
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 65C
Optimum temperature65 oC
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
BGOWY
50 - 7550 - 7525 - 5025 - 50100

References: Abdurashitov, M.A., Dedkov, V.S., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2000).