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SibEnzyme Ltd. :: Display product information: Zsp2 I
Zsp2 I
Catalog numberE145E146
Package, u.a.10005000
Concentration, u.a./ml5000-200005000-20000

Recognition siteATGCA^T
Methylation sensitivitynot tested
OriginZoogloea species 2
Storage conditions10 mM Tis-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.) + BSA

Enzyme % activity:
10050 - 7525 - 5025 - 5025 - 50

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Dedkov, V.S., Degtyarev, S.Kh., unpublished observations.