Go to sibenzyme.com
SibEnzyme Ltd. :: Display product information: Xma I
Xma I
Catalog numberE233E234
Package, u.a.3001500
Concentration, u.a./ml30003000

Recognition siteC^CCGGG
Methylation sensitivitynot tested
OriginE.coli strain, that carries the cloned gene XmaI from Xanthomonas malvacearum
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated. Of these 90% can be recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 3 u.a. of enzyme for 16 hours at 37C
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
75 - 10050 - 750 - 100 - 10100

References: Endow, S.A., Roberts, R.J. J. Mol. Biol. 112: 521-529 (1977).