|Catalog number||E141||E142||E141X||E142X |
|Package, u.a.||2000||10000||2000||10000 |
|Concentration, u.a./ml||20000||20000||50000||50000 |
|Methylation sensitivity||Blocked by overlapping Dam-methylation (GmATC): TCTAGATC|
|Origin||E.coli strain, that carries the cloned gene XbaI from Xanthomonas badrii|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C.|
|Assayed on||Lambda DNA (dam-/HindIII-digest)|
|Ligation||After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.|
|Non-specific activity||No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.|
|Optimum temperature||37 oC|
|Inactivation 20 minutes||65oC|
|Optimum SE-buffer||O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA|
Enzyme % activity:
|75 - 100||75 - 100||100||50 - 75||75 - 100|
Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Zain, B,S., Roberts, R.J. J. Mol. Biol. 115: 249-255 (1977).