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SibEnzyme Ltd. :: Display product information: Xba I
Name
Xba I
Catalog numberE141E142E141XE142X
Package, u.a.200010000200010000
Concentration, u.a./ml20000200005000050000

Recognition siteT^CTAGA
AGATC^T
Methylation sensitivityBlocked by overlapping Dam-methylation (GmATC): TCTAGATC
OriginE.coli strain, that carries the cloned gene XbaI from Xanthomonas badrii
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20C.
Assayed onLambda DNA (dam-/HindIII-digest)
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
75 - 10075 - 10010050 - 7575 - 100

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Zain, B,S., Roberts, R.J. J. Mol. Biol. 115: 249-255 (1977).