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SibEnzyme Ltd. :: Display product information: Vsp I
Vsp I
Catalog numberE139E140
Package, u.a.10005000
Concentration, u.a./ml1000010000

Recognition siteAT^TAAT
Methylation sensitivityBlocked by ATTAmAT methylation.
OriginE.coli strain, that carries the cloned gene VspI from Vibrio species 343
Storage conditions10 mM KPO4 (pH 7.2); 50 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme 70% of the DNA fragments can be ligated.Of these, 90% can be recut. In the presence of 10% PEG ligation is better.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
0 - 1010 - 2550 - 7510025 - 50

References: Degtyarev, S.Kh., Repin, V.E., Rechkunova, N.I., Tchigikov, V.E., Malygin, E.G., Mikhajlov, V.V., Rasskazov, V.A. Bioorg. Khim. 13: 420-421 (1987).