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SibEnzyme Ltd. :: Display product information: Vne I
Name
Vne I
Catalog numberE137E138
Package, u.a.10005000
Concentration, u.a./ml10000-2000010000-20000

Recognition siteG^TGCAC
CACGT^G
Methylation sensitivitynot tested
OriginVibrio nereis 18
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
10 - 2525 - 5010025 - 5025 - 50

References: Degtyarev, S.Kh., Rechkunova, N.I., Netesova, N.A., Tchigikov, V.E., Malygin, E.G., Kochkin, A.V., Mikhajlov, V.V., Rasskazov, V.A. Bioorg. Khim. 13: 422-423 (1987).