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SibEnzyme Ltd. :: Display product information: Tth111 I
Tth111 I
Catalog numberE097E098
Package, u.a.4002000
Concentration, u.a./ml50005000

Recognition siteGACN^NNGTC
Methylation sensitivitynot tested
OriginE.coli strain, that carries the cloned gene Tth111I from Thermus Thermophilus 111
Storage conditions10 mM Tris-HCl (pH 7.5); 500 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20C.
Assayed onLambda DNA (HindIII-digest)
LigationAfter 2-fold overdigestion with enzyme 10% of the DNA fragments can be ligated.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 5 u.a. of enzyme for 16 hours at 65C.
Optimum temperature65 oC
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
75 - 10050 - 7510 - 2510 - 25100

Note: High enzyme concentration may result in star activity.
References: Shinomiya, T., Sato, S. Nucleic Acids Res. 8: 43-56 (1980).