Go to sibenzyme.com
SibEnzyme Ltd. :: Display product information: Acs I
Name
Acs I
Catalog numberE013E014
Package, u.a.5002500
Concentration, u.a./ml10000-2000010000-20000

Recognition siteR^AATTY
YTTAA^R
Methylation sensitivitynot tested
OriginArthrobacter citreus
Storage conditions20 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 20-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 50 u.a. of enzyme for 16 hours at 50C.
Optimum temperature50 oC
Inactivation 20 minutes80oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
25 - 5050 - 7550 - 7510010 - 25

Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Degtyarev, S.Kh., Kolyhalov, A.A., Rechkunova, N.I., Abdurashitov, M.A., Nucleic Acids Res. 20: 3789 (1992).