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SibEnzyme Ltd. :: Display product information: Tru9 I
Name
Tru9 I
Catalog numberE199E200
Package, u.a.5002500
Concentration, u.a./ml2000020000

Recognition siteT^TAA
AAT^T
Methylation sensitivityBlocked by TTAmA methylation .
OriginE.coli strain that carries the cloned Tru9 I gene from from Thermus rubber 9
Storage conditions10 mM Tris-HCl-(pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol Store at -20C.
Assayed onLambda DNA
LigationAfter 20-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 40 u.a. of enzyme for 16 hours at 65C.
Optimum temperature65 oC
Inactivation 20 minutes80oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
75 - 10025 - 5025 - 5010050 - 75

References: Prichodko, E.A., Rechkunova, N.I., Degtyarev, S.Kh. Sib. Biol. J. 1:57-59 (1991).