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SibEnzyme Ltd. :: Display product information: Taq I
Name
Taq I
Catalog numberE133E134
Package, u.a.200010000
Concentration, u.a./ml2000020000

Recognition siteT^CGA
AGC^T
Methylation sensitivitynot tested
OriginE.coli strain, that carries the cloned gene Taq I from Thermus aquaticus
Storage conditions10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 20-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65C.
Optimum temperature65 oC
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
50 - 7575 - 10075 - 10050 - 75100

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Sato, S., Hutchison, C.A. III, Harris, J.I. Proc. Natl. Acad. Sci. U. S. A. 74: 542-546 (1977).