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SibEnzyme Ltd. :: Display product information: Ssp I
Name
Ssp I
Catalog numberE041E042
Package, u.a.5002500
Concentration, u.a./ml1000010000

Recognition siteAAT^ATT
TTA^TAA
Methylation sensitivityBlocked by methylation AmATATT.
OriginE.coli strain, that carries the cloned gene SspI from Sphaerotilus species
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferK (10 mM Tris-HCl (pH 7.6 at 250C); 10 mM MgCl2; 100 mM KCl; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
75 - 10050 - 7525 - 5050 - 7575 - 100

Note: High enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Schildkraut, I., Grandoni, R. unpublished observations.