|Catalog number||E041||E042 |
|Package, u.a.||500||2500 |
|Concentration, u.a./ml||10000||10000 |
|Methylation sensitivity||Blocked by methylation AmATATT.|
|Origin||E.coli strain, that carries the cloned gene SspI from Sphaerotilus species|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.|
|Assayed on||Lambda DNA|
|Ligation||After 10-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.|
|Non-specific activity||No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.|
|Optimum temperature||37 oC|
|Inactivation 20 minutes||65oC|
|Optimum SE-buffer||K (10 mM Tris-HCl (pH 7.6 at 250C); 10 mM MgCl2; 100 mM KCl; 1 mM DTT.) + BSA|
Enzyme % activity:
|75 - 100||50 - 75||25 - 50||50 - 75||75 - 100|
Note: High enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Schildkraut, I., Grandoni, R. unpublished observations.