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SibEnzyme Ltd. :: Display product information: Sse9 I
Name
Sse9 I
Catalog numberE217E218
Package, u.a.5002500
Concentration, u.a./ml50005000

Recognition site^AATT
TTAA^
Methylation sensitivitynot tested
OriginE.coli strain that carries the cloned Sse9 I gene from Sporosarcina species
Storage conditions10 mM Tis-HCl (pH 7.5); 50 mM NaCl; 10 mM MgCl2; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20C.
Assayed onpBR322 DNA
LigationAfter 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of DNA with 5 u.a. of enzyme for 16 hours at 55C.
Optimum temperature55 oC
Inactivation 20 minutes65oC
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
10075 - 10050 - 7550 - 7575 - 100

Note: At 37C activity is 75% from maximum.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Gonchar, D.A., Dedkov, V.S., Verkhozina, V.A., Kusner, Yu.S., Shevchenko, A.V., Degtyarev, S.Kh. Prikl. Biokhim. Mikrobiol. 34: 139-141 (1998).