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SibEnzyme Ltd. :: Display product information: SmiM I
Name
SmiM I
Catalog numberE293E294
Package, u.a.2001000
Concentration, u.a./ml5000-100005000-10000

Recognition siteCAYNN^NNRTG
GTRNN^NNYAC
Methylation sensitivitynot tested
OriginSphingobacterium mizutae M
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; and 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
10 - 2510 - 2575 - 10010010 - 25

References: Dedkov, V.S., Najakshina, T.N., Gushina, E.N., Abdurashitov, M.A., Degtyarev, S.Kh. Unpublished observations (1999).