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SibEnzyme Ltd. :: Display product information: Smi I
Smi I
Catalog numberE225E226
Package, u.a.10005000
Concentration, u.a./ml10000-3000010000-30000

Recognition siteATTT^AAAT
Methylation sensitivitynot tested
OriginStreptococcus milleri S
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onT7 DNA (SspI-digest)
LigationAfter 5-fold overdigestion with enzyme 80% of the DNA fragments can be ligated and recut. Ligation >95% in presence of 10% PEG.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of T7 DNA with 30 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
25 - 5025 - 5010075 - 10025 - 50

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Dedkov, V.S., Bondar, T.S., Shevchenco, A.V., Degtyarev, S.Kh. Mol. Gen. Mikrobiol. Virusol. 1: 23-27 (2000).