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SibEnzyme Ltd. :: Display product information: Sma I
Name
Sma I
Catalog numberE177E178
Package, u.a.200010000
Concentration, u.a./ml2000020000

Recognition siteCCC^GGG
GGG^CCC
Methylation sensitivitynot tested
OriginE.coli strain that carries the cloned Sma I gene from Serratia marcescens
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA (HindIII-digest)
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated (by using of high concentration T4 DNA Ligase and 10% PEG) and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 40 u.a. of enzyme for 16 hours at 25C.
Optimum temperature25 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
BGOWY
0 - 100 - 100 - 100 - 10100

References: Endow, S.A., Roberts, R.I., J. Mol. Biol. 112: 521-529 (1977).