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SibEnzyme Ltd. :: Display product information: Sfr274 I
Sfr274 I
Catalog numberE125E126
Package, u.a.200010000
Concentration, u.a./ml10000-3000010000-30000

Recognition siteC^TCGAG
Methylation sensitivityBlocked by CTCGmAG methylation.
Not blocked by CTmCGAG methylation.
OriginStreptomyces fradiae 274
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol.. Store at -20C.
Assayed onLambda DNA (Hind III-digest)
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 50C.
Optimum temperature50 oC
Inactivation 20 minutes65oC
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.)

Enzyme % activity:
10075 - 10050 - 7550 - 7575 - 100

References: Puchkova, L.I., Krivopalova, G.N., Andreeva, I.S., Selina, A.V., Serov, G.D., Rechkunova, N.I., Degtyarev, S.Kh. Izv. Sib. Otd. Akad. Nauk SSSR 1: 32-34 (1990).