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SibEnzyme Ltd. :: Display product information: AclW I
Name
AclW I
Catalog numberE211E212
Package, u.a.100500
Concentration, u.a./ml1000-30001000-3000

Recognition siteGGATC(N)4^
CCTAG(N)5^
Methylation sensitivityBlocked by Dam methylation (GmATC) GGATC
OriginAcinetobacter calcoaceticus W2131
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA (dam-)
LigationAfter 2-fold overdigestion with enzyme about 50% of the DNA fragments can be ligated with more than 500 u.a. of DNA ligase.
Non-specific activityNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 6 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
75 - 10050 - 750 - 100 - 10100

Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References: Abdurashitov, M.A., Belichenko, O.A., Shevchenko, A.V., Degtyarev, S.Kh. Unpublished observations. (1994)