Go to sibenzyme.com

Name AclW I Catalog number E211 E212 Package, u.a. 100 500 Concentration, u.a./ml 1000-3000 1000-3000 Recognition site GGATC(N)4^ CCTAG(N)5^ Methylation sensitivity Blocked by Dam methylation (GmATC) GGATC Origin Acinetobacter calcoaceticus W2131 Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C. Assayed on Lambda DNA (dam-) Ligation After 2-fold overdigestion with enzyme about 50% of the DNA fragments can be ligated with more than 500 u.a. of DNA ligase. Non-specific activity No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 6 u.a. of enzyme for 16 hours at 37°C. Optimum temperature 37 oC Inactivation 20 minutes 65oC Optimum SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA Enzyme % activity: B G O W Y 75 - 100 50 - 75 0 - 10 0 - 10 100 Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation. References: Abdurashitov, M.A., Belichenko, O.A., Shevchenko, A.V., Degtyarev, S.Kh. Unpublished observations. (1994)