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SibEnzyme Ltd. :: Display product information: SfaN I
Name
SfaN I
Catalog numberE165E166
Package, u.a.5002500
Concentration, u.a./ml1000010000

Recognition siteGCATC(N)5^
CGTAG(N)9^
Methylation sensitivitynot tested
OriginE.coli strain that carries the cloned SfaN I gene from Streptococcus faecalis N
Storage conditions10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
10 - 2525 - 5010075 - 1000 - 10

References: Schildkraut, I., Greenough, L. Unpublished observations.