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SibEnzyme Ltd. :: Display product information: Sbf I
Name
Sbf I
Catalog numberE101E102
Package, u.a.2001000
Concentration, u.a./ml50005000

Recognition siteCCTGCA^GG
GG^ACGTCC
Methylation sensitivitynot tested
OriginIsolated from Streptomyces species Bf61
Storage conditions10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol ; Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and of these more than 90% canrecut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
BGOWY
75 - 10050 - 750 - 100 - 10100

Note: High enzyme concentration may result in star activity.
References: Abdurashitov, M.A., Kileva, E.V., Shevchenko, A.V., Degtyarev, S.Kh. Unpublished observations (1994).