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SibEnzyme Ltd. :: Display product information: Sal I
Name
Sal I
Catalog numberE115E116
Package, u.a.200010000
Concentration, u.a./ml1000010000

Recognition siteG^TCGAC
CAGCT^G
Methylation sensitivitynot tested
OriginE.coli strain that carries the cloned Sal I gene from Streptomyces albus
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA (HindIII-digest)
LigationAfter 10-fold overdigestion with enzyme more than 95% of the DNA pUC19 fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
0 - 1010 - 2510025 - 500 - 10

Note: High enzyme concentration may result in star activity.
References: Arrand, J.R., Myers, P.A., Roberts, R.J., J. Mol. Biol. 118: 113-122 (1978).