Go to sibenzyme.com
SibEnzyme Ltd. :: Display product information: Rsr2 I
Name
Rsr2 I
Catalog numberE281E282
Package, u.a.10005000
Concentration, u.a./ml10000-3000010000-30000

Recognition siteCG^GWCCG
GCCWG^GC
Methylation sensitivitynot tested
OriginRhodobacter sphaeroides 12
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 ug/ml BSA, 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 60 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
50 - 7575 - 1000 - 1010 - 25100

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Shinkarenko, O.A., Lebedeva, N.A., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.Kh. Unpublished observations (1998).